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KMID : 0364219870300030219
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Korean Journal of Zoology
1987 Volume.30 No. 3 p.219 ~ p.230
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Evolutionary Study on the Dark Chub (Zacco temmincki) III. The Euect of Reaction Temperature on the Kinetic Mode of Isolated SMDH Isozymes from Zacco temmincki
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Kang Dong-Chul
Yang Suh-Yung
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Abstract
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Two allelotypes of sMDH variation, namely A and B type, are known in the dark chub, Zacco temmincki. We attempted to clarify their probable functional enzymatic difEerence with temperature change. Two types of sMDH were purified separately by successive chromatography on DEAE-cellulose and blue 2-Sepharose amEnity columns, and their ensymatic activities to temperature change were measured. Q10 of Vmax and Vmax/Km were significantly different between two types, i.e. A type being higher in Q10 values than B type. Based on the result it is assumed that A type may be more sensitive to temperature change than B type.
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